|
KMID : 1007520140230020609
|
|
Food Science and Biotechnology
2014 Volume.23 No. 2 p.609 ~ p.613
|
|
|
Selective Cytotoxicity of Saururus chinensis in Glucose-Deprived HT- 29 Human Colon Cancer Cells
|
|
Son Eun-Soon
Choi Jeong-Un
Lee Kyung-Jin
Park Hae-Ryong
|
|
|
Abstract
|
|
|
|
In this study, the selective cytotoxic activity of methanolic extracts of Saururus chinensis (SCE) was examined on glucose-deprived HT-29 cells. The effects of SCE on 2-deoxy-glucose (2DG) or glucose-free stressed HT-29 cells were evaluated through the MTT reduction assay, morphological observations, and the colony formation assay, in which SCE (5, 10 μg/mL) was found to be highly toxic to HT-29 cells only during glucose-deprived conditions. In addition, the mechanism of selective cytotoxic effects on SCE was assessed by Western blot analysis; SCE suppressed the accumulation of GRP78. Furthermore, apoptotic effects of SCE on glucose-deprived HT-29 cells were identified by Hoechst 33342 staining and flow cytometric analysis. In conclusion, SCE induced apoptosis in HT-29 cells under glucose-deprived conditions by down-regulation of GRP78. Cytotoxic effects on HT-29 cells were only observed during glucose deprivation, suggesting that SCE may be a target for novel therapeutic agents for treating colon cancer under glucose-deprived stress.
|
|
|
KEYWORD
|
|
|
Saururus chinensis, glucose deprivation, unfolded protein response, GRP78, HT-29 cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|